N2Jenomics Lab Pvt. Ltd. offers Full-Length 16S, 18S, and ITS Amplicon Sequencing using advanced PacBio SMRT long-read technology for accurate microbial identification and comprehensive microbiome profiling. Our service delivers species- and strain-level resolution, making it ideal for microbial taxonomy, diversity analysis, and phylogenetic studies.

What Is Full-Length 16S/18S/ITS Amplicon Sequencing?

Full-length 16S/18S/ITS amplicon sequencing is a long-read sequencing approach used to characterize bacterial, archaeal, fungal, and other eukaryotic microbial communities.

The workflow involves DNA extraction, amplification of the complete 16S rRNA, 18S rRNA, or ITS region using universal primers, followed by long-read sequencing. Sequencing the full-length marker improves taxonomic resolution, enabling more accurate species and strain identification compared with partial amplicon sequencing.

Why Choose Full-Length Amplicon Sequencing?

Full-length sequencing provides greater resolution than conventional short-read methods, allowing researchers to better characterize microbial diversity, community composition, and phylogenetic relationships. The resulting high-quality data also supports multi-omics integration and ecological studies.

Key Advantages

• • Full-length long reads for improved taxonomic resolution.

• • High-accuracy species and strain identification.
• • Comprehensive microbial community profiling.
• • PacBio SMRT sequencing for reliable long-read performance.
• • High-quality CCS reads for enhanced sequencing accuracy.
• • Robust bioinformatics analysis with publication-ready results.
• • Fast turnaround times and competitive pricing.

Applications

• Medical Research

Characterize human microbiomes and investigate microbial associations with health and disease.

• Animal Science

Study gut and rumen microbiota to understand animal nutrition, health, and productivity.

• Agricultural Research

Analyze rhizosphere microbial communities, plant–microbe interactions, and soil health.

• Environmental Microbiology

Investigate microbial populations involved in wastewater treatment, bioremediation, marine ecosystems, and environmental monitoring.

• Extreme Environment Studies

Explore microbial diversity and adaptation in extreme habitats such as hot springs, deep-sea environments, and acidic or saline ecosystems.

Workflow of Full-length 16S/18S/ITS Amplicon Sequencing

Service Specification

	Sample Requirements: Genomic DNA ≥ 500ng, concentration≥10 ng/µL, OD260/280=1.8-2.0 Tissue: 1-3g, Minimum Quantity: 1g Thallus: 5 g, Minimum Quantity: 3 g Interstitial Fluid: 3-5 mL, Minimum Quantity:1 mL Environmental Samples: 3-5g, Minimum Quantity: 1 g Water filter membrane: 3, Minimum Quantity: 1 PCR Products ≥400 ng
	Sequencing: PacBio Sequel Platforms 2000~20000 CCS reads/per sample
	Data Analysis OTUs clustering and filtering OTUs analysis and species annotation PCA, Venn diagram. A rank curve will be generated based on OTU abundance Alpha diversity, Beta diversity, Meta-analysis Multi-variate statistical analysis … (more upon request)

Bioinformatics Pipeline

Deliverables

• • The original sequencing data
• • Experimental results
• • Data analysis report
• • Details in Full-Length 16S/18S/ITS Amplicon Sequencing for your writing (customization)

1. What are the advantages of third-generation amplicon sequencing?

Third-generation sequencing using PacBio SMRT technology generates full-length amplicons with long, high-accuracy reads. This enables more precise microbial identification, improved taxonomic resolution, and better species- and strain-level classification than short-read sequencing.

2. Why choose full-length 16S/18S/ITS sequencing?

Unlike short-read methods that sequence only selected variable regions, full-length sequencing captures the entire 16S, 18S, or ITS region. This reduces primer bias, improves taxonomic accuracy, and provides more reliable comparisons across microbial communities.

3. How are bacterial species identified?

Species identification is performed by comparing full-length 16S rRNA sequences against curated reference databases. High sequence similarity supports accurate taxonomic classification, while additional genomic or phenotypic analyses may be used for further confirmation when needed.

4. What are the sample requirements?

• • Cultured microorganisms: Pure cultures with appropriate growth information are recommended.

• • Genomic DNA: Minimum 500 ng of high-quality DNA is generally required.
• • Gram-positive bacteria: Purified genomic DNA is recommended to ensure optimal sequencing results.

5. What is the typical length of 16S, 18S, and ITS sequences?

• • 16S rRNA: Approximately 1,500 bp, commonly used for bacterial and archaeal identification.
• • 18S rRNA: Approximately 1,500–2,000 bp, widely used for eukaryotic microbial classification.
• • ITS region: Typically 300–800 bp (species-dependent), the preferred marker for fungal identification and diversity analysis due to its high interspecies variability.