PacBio SMRT Sequencing Services

Long-Read Sequencing for High-Resolution Genomic Analysis

N2Jenomics Lab offers advanced PacBio Single Molecule Real-Time (SMRT) Sequencing services for researchers seeking highly accurate long-read sequencing solutions. PacBio SMRT technology generates long, high-fidelity (HiFi) reads that enable comprehensive analysis of complex genomes, structural variants, full-length transcripts, and epigenetic modifications with exceptional accuracy.

Unlike conventional short-read sequencing platforms, PacBio SMRT sequencing reads individual DNA molecules in real time without PCR amplification bias, providing improved coverage across repetitive, GC-rich, and structurally complex genomic regions.

Why Choose PacBio SMRT Sequencing?

PacBio SMRT sequencing provides several advantages for genomic and transcriptomic research:

• Long-read sequencing for improved genome assembly
• High-accuracy HiFi reads with excellent consensus quality
• Accurate detection of structural variants (SVs), insertions, deletions, and copy number variations (CNVs)
• Resolution of repetitive and GC-rich genomic regions
• Full-length transcript sequencing without transcript assembly
• Direct detection of DNA methylation and other epigenetic modifications
• Reduced amplification bias through single-molecule sequencing

Our PacBio SMRT Sequencing Workflow

N2Jenomics Lab follows a comprehensive workflow designed to ensure reliable sequencing results:

1. Sample quality assessment
2. High-molecular-weight DNA or RNA quality control
3. SMRTbell library preparation
4. PacBio sequencing
5. Raw data quality assessment
6. Bioinformatics analysis
7. Comprehensive data reporting and interpretation

Applications

Our PacBio SMRT sequencing services support a broad range of research applications, including:

Whole Genome Sequencing

Generate highly contiguous genome assemblies with superior resolution of repetitive sequences and structural variants.

De Novo Genome Assembly

Construct reference-quality genomes for microbial, plant, animal, and human research without dependence on existing reference genomes.

Structural Variant Analysis

Identify large insertions, deletions, inversions, duplications, translocations, and other complex genomic rearrangements that are often difficult to detect using short-read sequencing.

Full-Length RNA Sequencing (Iso-Seq)

Capture complete transcript isoforms in a single read to accurately characterize alternative splicing events, novel transcripts, fusion genes, and transcript diversity.

Epigenetic Analysis

Simultaneously analyze DNA sequence and methylation patterns without the need for bisulfite conversion, enabling deeper insights into epigenetic regulation.

Metagenomics

Improve microbial genome reconstruction and species-level identification within complex microbial communities.

Bioinformatics Analysis

Our experienced bioinformatics team provides end-to-end analysis, including:

• Raw data quality assessment
• Genome assembly
• Variant calling
• Structural variant detection
• SNP and InDel analysis
• Haplotype phasing
• DNA methylation analysis
• Transcript isoform characterization
• Functional annotation
• Customized downstream analysis based on project requirements

Deliverables

Clients receive a complete project package that may include:

• Raw sequencing data (FASTQ/BAM)
• Quality control reports
• Genome assembly files (where applicable)
• Variant analysis reports
• Structural variant summaries
• Transcriptome analysis reports
• Bioinformatics documentation
• Final project report with interpretation
• Customized data formats upon request

Why Partner with N2Jenomics Lab?

At N2Jenomics Lab, we combine advanced sequencing technologies with experienced molecular biologists and bioinformatics specialists to deliver reliable, reproducible, and high-quality genomic data. Our customized workflows support academic research, biotechnology, agriculture, microbiology, precision medicine, and pharmaceutical discovery projects.

Whether your project involves complex genome assembly, transcriptome characterization, structural variation analysis, or epigenomic research, our PacBio SMRT Sequencing solutions are designed to provide comprehensive and actionable genomic insights.